The coast to coast challenge begins at Morecambe Bay then across the Yorkshire Dales peaking at 337 metres above sea level as the riders pass through Sedbergh and Leyburn, with an overnight stay at Middleham.
Day two passes through the North Yorkshire Moors including the steep climbs and descents of Sutton Bank before finishing in Scarborough, a total of 131 miles (211 kilometres) later.
Brian Mills, duty station manager, Red Watch, said: “We were sold on the idea of the Coast to Coast challenge as a fund raiser by fire fighter, Adrian Gosnay, a keen cyclist on our team who has also designed the route.
"All of the fire fighters taking part are taking the challenge very seriously and we will certainly have to work hard to finish.
“Every donation small or large really does make a difference to the thousands of children who visit Sheffield Children’s Hospital. Our aim is to smash our £1,000 target with the support of sponsors and would ask people to visit our Just Giving page and help us get there.”
Doncaster Sheffield Airport (DSA) is an avid supporter of the charity, having already raised over £15,000 on behalf of The Children’s Hospital Charity and it will also be putting up a prize for one lucky donor to win return flights for two people to any one of its Flybe destinations.
The Children’s Hospital Charity mascot, Theo the bear, joined one of the training sessions to cheer along the fire officers, and offer some encouragement for their coast to coast challenge on behalf of this worthwhile cause.